Ionization vacuum gauge with all but the end of the ion collector shielded Patent

Describing hot filament type Bayard-Alpert ionization gage with ion collector buried or removed from grid structur

Quantifying the Differences in Binding Affinity of Reduced Glutathione for Glutathione S-Transferase at pH 6.5 and 8.5 Using Isothermal Titration Calorimetry

The binding affinity between an enzyme and its substrate is often dependent on the pH of the local environment. Knowing the pH at which reduced glutathione (GSH) binds with the highest affinity to the enzyme glutathione S-transferase (GST) is useful for determining the optimal pH for purification of GST-fusion proteins during GST-affinity chromatography. In this study, GST of the species Schistosoma japonicum was purified, quantified, and utilized to study its binding interaction with GSH at pH 6.5 and 8.5 via isothermal titration calorimetry (ITC). After protein expression, extraction, and purification, the GST concentration was quantified using QubitTM fluorometry. Thermodynamic properties and a dissociation constant (KD) for each experiment were obtained utilizing the MicroCal PEAQ-ITC Analysis Software for the binding of GSH to GST at pH 6.5 and 8.5. Statistical analysis of the technical replicate data was performed to obtain an average and standard deviation of the KD at each pH point. The results indicate a statistically significant difference (

Analytical study of the optimum geometric configuration of a space shuttle materials laboratory

A steady state, collisionless flow analysis was made of the density distribution within a hemisphere-disc system due to independent, uniformly distributed internal gas sources. The model was used to estimate the density within a molecular shield, deployed from the shuttle orbiter, which contained internal experiments having a prescribed gas source. Contour plots of the density distribution within the system were presented for disc-to-hemisphere radius ratios of .1, .3, .5, .7, and for disc-to-hemisphere surface emission flux density ratios of .01, 1, 100. The hemisphere-disc system was compared to the empty hemisphere, and it was found that if the disc emission flux density was the same as the hemisphere and the disc radius was not greater than 1/3 of the hemisphere radius, the increase in density at the center of the hemisphere-disc system was less than 50%

Problems in the Insulin Dependent Diabetic

It is often useful for the physician to classify diabetes as stable or unstable. Maturity-onset diabetes is usually stable, and its management is not difficult, provided the patient is interested and cooperative. Tendency to obesity, relative insensitivity to insulin, and absence of ketosis are characteristic of such patients. Relative insensitivity to insulin does not imply unresponsiveness, but rather that the blood sugar is not overly labile and does not fall sharply in response to exercise or injected insulin. Many patients with such insensitivity to insulin are adequately managed on diet, alone or in combination with the oral hypoglycemic agents. When the latter fail, usually a single morning dose of long-acting insulin is satisfactory in controlling glycosuria and hyperglycemia during a 24-hour interval. Occasionally a small dose of crystalline insulin given in the same injection will be needed to control glycosuria between breakfast and lunch